Gene technology for research, food, therapeutic cloning, and…

Gene technology for research, food, therapeutic cloning, and bioengineering. How far should we go? Watch the and discuss the ethical guidelines you would think are needed (narrow or wide, innovation enabling) based on our ability to genetically modify organisms (GMO), cloning animals or growing synthetic tissues in the laboratory. Its hard to find an unbiased approach to this kind of discussions, I think there are less ethical guidelines when it comes to growing the organs in a lab, since the donations of organs and cell extractions are done in a legal and ethical way. However, xenotransplantation ( growing organs in other organism to later transplant it) might sound ethically compromising, since we are farming the organs from other organism such as pigs. Where as an article from the NewYork Times magazine states : “Topics include the use of pig heart valves to replace leaky and hardened human heart, the incompatibility of the immune system during transplantation and the birth of the first pig without endogenous viruses through editing of the genetically normal cells of a living pig.” I personally think that, farms that breed pigs for consumption don’t really follow ethical guidelines in order to maximize their profits, and sadly I don’t see that xenotransplantation will be any different. 1. Distinguish between a familial and a sporadic cancer 2. It is often the case that a predisposition to certain forms of cancer is inherited. An example is familial retinoblastoma. What does it mean to have inherited an increased probability of acquiring a certain form of cancer? What subsequent event(s) must occur? 3. What are the roles of cellular proto-oncogenes, and how are these roles consistent with their implication in oncogenesis? 4. In DNA repair, how does the normal allele of BRCA1 work? Is it an oncogene or a tumor suppressor gene? 1. Cloning is a general term used for whole organisms and DNA sequences. Define what we mean when we say we have a clone. 2. The following DNA sequence contains a six-base sequence that is a recognition and cutting site for a restriction enzyme. What is the recognition sequence? Which enzyme will cut this sequence and where in the sequence? 5’ CCGAGAAGCTTAC 3’ 3’ GGCTCTTCGAATG 5’ 3. In cloning human DNA, why is it necessary to insert the DNA into a vector such as a bacterial plasmid? 4. Briefly describe the steps necessary to clone a human gene (i.e. make it recombinant DNA) with multiple introns, so it can be expressed in a bacterial cell.

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